Adenoviral vector-mediated GDNF gene transfer prevents death of adult facial motoneurons

Citation
T. Sakamoto et al., Adenoviral vector-mediated GDNF gene transfer prevents death of adult facial motoneurons, NEUROREPORT, 11(9), 2000, pp. 1857-1860
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROREPORT
ISSN journal
0959-4965 → ACNP
Volume
11
Issue
9
Year of publication
2000
Pages
1857 - 1860
Database
ISI
SICI code
0959-4965(20000626)11:9<1857:AVGGTP>2.0.ZU;2-Q
Abstract
We examined neuroprotective effects of an adenoviral vector encoding glial cell line-derived neurotrophic factor (Ax-CAhGDNF) on the lesioned adult ra t facial motoneurons. After facial nerve avulsion, animals locally injected into the facial canal with AxCALacZ (adenovirus encoding beta-galactosidas e gene) or AxCAhGDNF showed expression of beta-galactosidase activity or in tense immunolabeling for GDNF in lesioned facial motoneurons, respectively. The treatment with AxCAhGDNF after avulsion significantly prevented the lo ss of lesioned facial motoneurons, ameliorated choline acetyltransferase im munoreactivity, and suppressed the activity of nitric oxide synthase in the se neurons. These results indicate that the adenovirus-mediated gene transf er of GDNF may prevent the degeneration of motoneurons in adult humans with peripheral nerve injury and motor neuron diseases. NeuroReport 11:1857-186 0 (C) 2000 Lippincon Williams & Wilkins.