Autoantibodies to DFS 70 kd/transcription coactivator p75 in atopic dermatitis and other conditions

Citation
Rl. Ochs et al., Autoantibodies to DFS 70 kd/transcription coactivator p75 in atopic dermatitis and other conditions, J ALLERG CL, 105(6), 2000, pp. 1211-1220
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
ISSN journal
0091-6749 → ACNP
Volume
105
Issue
6
Year of publication
2000
Part
1
Pages
1211 - 1220
Database
ISI
SICI code
0091-6749(200006)105:6<1211:ATD7KC>2.0.ZU;2-A
Abstract
Background: Sera of patients with atopic dermatitis (AD) were found to have autoantibodies that reacted with tissue culture cell substrates in immunoh istochemistry to display a characteristic pattern of nuclear distribution o f dense fine speckles. The sera also recognized a 70-kd protein on Western immunoblots, and the antigen was termed dense fine speckles 70 kd (DSF70). Objective: Because spontaneously occurring autoantibodies could be immune r esponses to proteins that might be participating in the disease process, it was of interest to identify the antigens driving the autoimmune antibody r esponse. Methods: A serum containing high-titer antibodies to DFS70 was used to immu noscreen a complementary (c)DNA expression library to isolate cDNA encoding the antigen. After the cDNA was isolated, this was used to express recombi nant protein to determine the prevalence of antibody in AD and other condit ions. Results: Thirty percent of patients with AD were found to have antibody to recombinant DFS70 in Western immunoblots. Sixteen percent of patients with asthma and 9% of patients with interstitial cystitis had antibodies of the same specificities. The cDNA encoding DFS70 was identical to a transcriptio n coactivator called p75, which had been shown to be required for RNA polym erase II-dependent transcription. Another important finding was that IgE an tibodies to DFS70 were also present in AD sera. Conclusion: It is suggested that a common basis for the presence of autoant ibodies to DFS70 might be related to AD in asthma, interstitial cystitis, a nd other conditions. A possible role of this antigen-antibody system in pat hogenesis remains to be demonstrated, but it appears to be a marker for a s ubset of patients with AD.