Structure and biological activities of eumenine mastoparan-AF (EMP-AF), a new mast cell degranulating peptide in the venom of the solitary wasp (Anterhynchium flavomarginatum micado)

Citation
K. Konno et al., Structure and biological activities of eumenine mastoparan-AF (EMP-AF), a new mast cell degranulating peptide in the venom of the solitary wasp (Anterhynchium flavomarginatum micado), TOXICON, 38(11), 2000, pp. 1505-1515
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
TOXICON
ISSN journal
0041-0101 → ACNP
Volume
38
Issue
11
Year of publication
2000
Pages
1505 - 1515
Database
ISI
SICI code
0041-0101(200011)38:11<1505:SABAOE>2.0.ZU;2-Y
Abstract
A new mast cell degranulating peptide, eumenine mastoparan-AF (EMP-AF), was isolated from the venom of the solitary wasp Anterhynchium flavomarginatum micado, the most common eumenine wasp found in Japan. The structure was an alyzed by FAB-MS/MS together with Edman degradation, which was corroborated by solid-phase synthesis. The sequence of EMP-AF, Ile-Asn-Leu-Leu-Lys-Ile- Ala-Lys-Gly-Ile-lle-Lys-Ser-Leu-NH2, was similar to that of mastoparan, a m ast cell degranulating peptide from a hornet venom; tetradecapeptide with C -terminus amidated and rich in hydrophobic and basic amino acids. In fact, EMP-AF exhibited similar activity to mastoparan in stimulating degranulatio n from rat peritoneal mast cells and RBL-2H3 cells. It also showed signific ant hemolytic activity in human erythrocytes. Therefore, this is the first example that a mast cell degranulating peptide is found in the solitary was p venom. Besides the degranulation and hemolytic activity, EMP-AF also affe cts on neuromuscular transmission in the lobster walking leg preparation. T hree analogs EMP-AF-1 similar to 3 were snythesized and biologically tested together with EMP-AF, resulting in the importance of the C-terminal amide structure for biological activities. (C) 2000 Elsevier Science Ltd. All rig hts reserved.