Treatment of cells with detergent activates caspases and induces apoptoticcell death

Citation
W. Strupp et al., Treatment of cells with detergent activates caspases and induces apoptoticcell death, J MEMBR BIO, 175(3), 2000, pp. 181-189
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF MEMBRANE BIOLOGY
ISSN journal
0022-2631 → ACNP
Volume
175
Issue
3
Year of publication
2000
Pages
181 - 189
Database
ISI
SICI code
0022-2631(20000601)175:3<181:TOCWDA>2.0.ZU;2-8
Abstract
Due to their amphiphilic properties, detergents readily disrupt cellular me mbranes and cause rapid cytolysis. In this study we demonstrate that treatm ent of cells with sublytic concentrations of detergents such as Triton X-10 0, Nonidet- P-30, n-octylglucoside and the bile salt sodium deoxycholate in duce typical signs of apoptosis including DNA fragmentation and cleavage of poly(ADP-ribose) polymerase molecules. The detergent concentration require d for apoptosis was below the critical micellar concentration. Induction of apoptosis was not restricted to human cells but similarly occurred in a va riety of other vertebrate cell lines. Unstimulated peripheral blood mononuc lear cells were susceptible to apoptosis induction by detergent suggesting that apoptosis in this circumstance is not mediated by CD95. Cell death was not due to influx of calcium from the medium. Apoptosis was blocked and cy tolysis prevented by treatment with peptide inhibitors of caspases. These f indings suggest a process of apoptosis that is initiated upon nonspecific a lterations at the cell membrane level. Physiologic correlates of this proce ss still have to be defined.