Analysis of the diversity of a sheep antibody repertoire as revealed from a bacteriophage display library

Citation
Ka. Charlton et al., Analysis of the diversity of a sheep antibody repertoire as revealed from a bacteriophage display library, J IMMUNOL, 164(12), 2000, pp. 6221-6229
Citations number
47
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
0022-1767 → ACNP
Volume
164
Issue
12
Year of publication
2000
Pages
6221 - 6229
Database
ISI
SICI code
0022-1767(20000615)164:12<6221:AOTDOA>2.0.ZU;2-N
Abstract
We have applied bacteriophage display technology to construct and analyze t he diversity of an IgG library of >1 x 10(8) clones from an adult sheep imm unized against the hapten atrazine. We have identified eight new V-H gene f amilies (V(H)2-V(H)9) and five new V kappa gene families (V kappa V-V kappa IX). The heavy and kappa light chain variable region gene loci were found to be far more diverse than previously thought.