Amyloid-beta 25-35 peptide induces expression of monoamine oxidase B in cultured rat astrocytes

Citation
W. Song et al., Amyloid-beta 25-35 peptide induces expression of monoamine oxidase B in cultured rat astrocytes, ACT PHAR SI, 21(6), 2000, pp. 557-563
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ACTA PHARMACOLOGICA SINICA
ISSN journal
0253-9756 → ACNP
Volume
21
Issue
6
Year of publication
2000
Pages
557 - 563
Database
ISI
SICI code
0253-9756(200006)21:6<557:A2PIEO>2.0.ZU;2-8
Abstract
AIM: To investigate the effects of amyloid-beta 25 - 35 peptide (A beta 25 - 35) on monoamine oxidase (MAO) expression and activity in primary culture d rat astrocytes. METHODS: Immunocytochemistry was used to observe the morp hological changes in astrocytes. Fluorescence spectrophotometry was used to measure the activity of MAO in astrocytes. The expression of MAO in astroc ytes was assayed by RT-PCR. RESULTS: A beta 25 - 35 induced a reactive morp hological change in cultured rat astrocytes which was accompanied by increa sed immunoreactivities for glial fibrillary acidic protein. Treatment with A beta 25 - 35 resulted in an elevation of MAO activity in a dose- and time -dependent manner. A beta 25 - 35-induced enhancement of MAO activity was o f type B (MAO-B). The increase in MAO-B activity appeared to be due to an i ncrease in the number of enzyme molecules since kinetic analysis demonstrat ed a 1.5 fold increase in V-max with no change in K-m. Treatment with A bet a 25 - 35 also led to a substantial increase in MAO-B mRNA level in the ast rocytes. CONCLUSION: A beta 25 - 35 is able to selectively induce MAO-B exp ression in rat astrocytes and that the upregulation of MAO-B in A beta 25 - 35-stimulated astrocytes may play an important role in the pathogenesis of Alzheimer's disease.