Cytokine-mediated differential regulation of macrophage activator protein-1 genes

Citation
Ts. Tengku-muhammad et al., Cytokine-mediated differential regulation of macrophage activator protein-1 genes, CYTOKINE, 12(6), 2000, pp. 720-726
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
CYTOKINE
ISSN journal
1043-4666 → ACNP
Volume
12
Issue
6
Year of publication
2000
Pages
720 - 726
Database
ISI
SICI code
1043-4666(200006)12:6<720:CDROMA>2.0.ZU;2-M
Abstract
The regulation of macrophage activator protein-1 (AP-1) gene expression by LPS and cytokines is of potentially crucial importance in the pathogenesis of several diseases. The action of LPS and four cytokines on AP-1 gene expr ession in the murine macrophage J774.2 cell line was, therefore, studied, E xposure of the cells to IL-6 produced no changes in the mRNA levels of all AP-1 members studied. In contrast, the expression of JunB, c-jun and c-fos, but not JunD, was increased by LPS, TNF-alpha, IFN-gamma and IL-1, albeit with different kinetics and magnitude of induction. Electrophoretic mobilit y shift assays showed a close correlation between the expression of the AP- 1 genes and the functional AP-1 DNA binding activity and, additionally, dem onstrated the participation of heterodimeric interactions between the diffe rent members. These studies provide insights into the potential mechanisms that may be involved in the mediator-specific modulation of AP-1 regulated macrophage gene expression. (C) 2000 Academic Press.