Electrophoretic behavior study and determination of some active componentsin Chinese medicinal preparations by capillary electrophoresis

Citation
Ht. Liu et al., Electrophoretic behavior study and determination of some active componentsin Chinese medicinal preparations by capillary electrophoresis, ANALYST, 125(6), 2000, pp. 1083-1086
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYST
ISSN journal
0003-2654 → ACNP
Volume
125
Issue
6
Year of publication
2000
Pages
1083 - 1086
Database
ISI
SICI code
0003-2654(2000)125:6<1083:EBSADO>2.0.ZU;2-K
Abstract
The determination of icariin (IC), rhein (RH), chrysophanol (CH), physcion (PHY), glycyrrhetic acid (GE), and glycyrrhizic acid (GI), in traditional C hinese preparations, Anshen Bunao oral liquid and Maren pill, has been inve stigated by micellar electrokinetic capillary electrophoresis. With borate buffer (10 mM), SDS (20 mM) and acetonitrile (10%) as background electrolyt e (pH 9.55), 20 kV applied voltage and 254 nm UV detection, the six active compounds were completely separated within 10 min. The effects of buffer pH , concentration of borate, SDS and modifier on electrophoretic behavior and separation are discussed. Regression equations revealed linear relationshi ps (correlation coefficients: 0.9960-0.9999) between the peak-area of each component and the content. In addition, the levels of the six active compou nds in two kinds of traditional Chinese medicinal preparations were easily determined with recoveries of from 94.7% to 106.4%.