Direct activation of capsaicin receptors by products of lipoxygenases: Endogenous capsaicin-like substances

Citation
Sw. Hwang et al., Direct activation of capsaicin receptors by products of lipoxygenases: Endogenous capsaicin-like substances, P NAS US, 97(11), 2000, pp. 6155-6160
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
0027-8424 → ACNP
Volume
97
Issue
11
Year of publication
2000
Pages
6155 - 6160
Database
ISI
SICI code
0027-8424(20000523)97:11<6155:DAOCRB>2.0.ZU;2-U
Abstract
Capsaicin, a pungent ingredient of hot peppers, causes excitation of small sensory neurons, and thereby produces severe pain. A nonselective cation ch annel activated by capsaicin has been identified in sensory neurons and a c DNA encoding the channel has been cloned recently. However, an endogenous a ctivator of the receptor has not yet been found. In this study, we show tha t several products of lipoxygenases directly activate the capsaicin-activat ed channel in isolated membrane patches of sensory neurons. Among them, 12- and 15-(S)-hydroperoxyeicosatetraenoic acids, 5- and 15-(S)-hydroxyeicosat etraenoic acids, and leukotriene Bq possessed the highest potency. The eico sanoids also activated the cloned capsaicin receptor (VR1) expressed in HEK cells. Prostaglandins and unsaturated fatty acids failed to activate the c hannel. These results suggest a novel signaling mechanism underlying the pa in sensory transduction.