Cell-to-cell movement of TMV RNA is temperature-dependent and corresponds to the association of movement protein with microtubules

Citation
V. Boyko et al., Cell-to-cell movement of TMV RNA is temperature-dependent and corresponds to the association of movement protein with microtubules, PLANT J, 22(4), 2000, pp. 315-325
Citations number
55
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
22
Issue
4
Year of publication
2000
Pages
315 - 325
Database
ISI
SICI code
0960-7412(200005)22:4<315:CMOTRI>2.0.ZU;2-P
Abstract
The movement protein (MP) of tobacco mosaic virus (TMV) is essential for sp read of the viral RNA genome from cell to cell. During infection, the MP as sociates with microtubules, and it has been proposed that the cytoskeleton transports the viral ribonucleoprotein complex from ER sites of synthesis t o plasmodesmata through which infection spreads into adjacent cells. Howeve r, microtubule association of MP was observed in cells undergoing late infe ction rather than in cells undergoing early infection at the leading edge o f expanding infection sites where virus RNA cell-to-cell spread occurs. The refore, alternative roles for microtubules in virus infection have been pro posed, including a role in MP degradation. To further investigate the role of microtubules in virus pathogenesis, we tested the efficiency of cell-to- cell spread of infection and microtubule association of the MP in response to changes in temperature. We show that the subcellular distribution of MP is temperature-dependent and that a higher efficiency of intercellular tran sport of virus RNA at elevated temperatures corresponds to an increased ass ociation of MP with microtubules early in infection.