Phylogenetic identification of hypermastigotes, Pseudotrichonympha, Spirotrichonympha, Holomastigotoides, and parabasalian symbionts in the hindgut of termites

Citation
M. Ohkuma et al., Phylogenetic identification of hypermastigotes, Pseudotrichonympha, Spirotrichonympha, Holomastigotoides, and parabasalian symbionts in the hindgut of termites, J EUKAR MIC, 47(3), 2000, pp. 249-259
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN journal
1066-5234 → ACNP
Volume
47
Issue
3
Year of publication
2000
Pages
249 - 259
Database
ISI
SICI code
1066-5234(200005/06)47:3<249:PIOHPS>2.0.ZU;2-B
Abstract
The phylogenetic diversity of parabasalian flagellates was examined based o n the sequences of small subunit ribosomal RNA genes amplified directly fro m the mixed population of flagellates in the hindgut of lower termites. In total, 33 representative sequences of parabasalids were recovered from eigh t termite species. Fluorescent-labeled oligonucleotide probes specific for certain sequences were designed and used for the in situ identification of parabasalian species by whole-cell hybridization. The hypermastigotes, Pseu dotrichonympha grassii. Spirotrichonympha leidyi, and Holomastigotoides mir abile in the hindgut of Coptotermes formosanus, and Spirotrichonympha sp. a nd Trichonympha spp. in Hodotermopsis sjoestedti were identified. In the ph ylogenetic tree constructed, the sequences from the termites were dispersed within the groups of known members of parabasalids, reflecting the presenc e of diverse parabasalids in the hindgut of termites. There were three para phyletic lineages of hypermastigotes represented by Pseudotrichonympha, Tri chonympha, and Spirotrichonympha, in agreement with the morphology-based ta xonomic groups. The analysis of the tree-root suggested that the Pseudotric honympha group is the most probable ancient lineage of parabasalids and tha t the Trichonympha group is the secondly deep-branching lineage. The Spirot richonympha group and the Trichomonadida may have emerged later.