A novel protein with RNA-binding motifs interacts with ataxin-2

Citation
H. Shibata et al., A novel protein with RNA-binding motifs interacts with ataxin-2, HUM MOL GEN, 9(9), 2000, pp. 1303-1313
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
HUMAN MOLECULAR GENETICS
ISSN journal
0964-6906 → ACNP
Volume
9
Issue
9
Year of publication
2000
Pages
1303 - 1313
Database
ISI
SICI code
0964-6906(20000522)9:9<1303:ANPWRM>2.0.ZU;2-9
Abstract
Spinocerebellar ataxia type 2 (SCA2) is caused by expansion of a polyglutam ine tract in ataxin-2, a protein of unknown function. Using the yeast two-h ybrid system, we identified a novel protein, A2BP1 (ataxin-2 binding protei n 1) which binds to the C-terminus of ataxin-2. Northern blot analysis show ed that A2BP1 was predominantly expressed in muscle and brain. By immunocfl uorescent staining, A2BP1 and ataxin-2 were both localized to the trans-Gol gi network. Immunocytochemistry showed that A2BP1 was expressed in the cyto plasm of Purkinje cells and dentate neurons in a pattern similar to that se en for ataxin-2 labeling. Western blot analysis of subcellular fractions in dicated enrichment of A2BP1 in the same fractions as ataxin-2, Sequence ana lysis of the A2BP1 cDNA revealed an RNP motif that is highly conserved amon g RNA-binding proteins. A2BP1 had striking homology with a human cDNA clone , P83A20, of unknown function and at least two copies of A2BP1 homologs are found in the Caenorhabditis elegans genome database. A2BP1 and related pro teins appear to form a novel gene family sharing RNA-binding motifs.