Amphipathic structure of theonellapeptolide-Id, a hydrophobic tridecapeptide lactone from the Okinawa marine sponge Theonella swinhoei

Doi, M Ishida, T Kobayashi, M Katsuya, Y Mezaki, Y Sasaki, M Terashima, A Taniguchi, T Tanaka, C

M. Doi et al., Amphipathic structure of theonellapeptolide-Id, a hydrophobic tridecapeptide lactone from the Okinawa marine sponge Theonella swinhoei, BIOPOLYMERS, 54(1), 2000, pp. 27-34

18

INGLESE

Article

Biochemistry & Biophysics

BIOPOLYMERS

0006-3525 → ACNP

54

1

2000

27 - 34

ISI

0006-3525(2000)54:1<27:ASOTAH>2.0.ZU;2-D

Theonellapeptolide-Id (TNLP), a cyclic tridecapeptide lactone, was crystall ized from dimethylformamide-water solution. In the asymmetric unit, two pep tide molecules were combined with solvent molecules, and the total molecula r weight was over 3000 Dalton. The crystal structure including solvent mole cules was finally determined at 0.80 Angstrom resolution using synchrotron radiation. The conformations of two independent molecules were similar to e ach other and were also similar to the previously reported structure (Doi. Ishida, Kobayashi, Deschamps and Filppen-Anderson. 1999. Acta Crystallogr S ect C, 55, 796-798). About 13 hydrated water molecules were found at disord ered 19 sites; they were located at a certain region to avoid contact with aliphatic side-chains of peptolide in the crystal. The spatial disposition of the solvent molecules and peptides subsequently caused the formation of the amphipathic layer. (C) 2000 John Wiley Be Sons, Inc. Biopoly 54: 27-34, 2000.