Improved production of potato microtubers using a temporary immersion system

Citation
E. Jimenez et al., Improved production of potato microtubers using a temporary immersion system, PL CELL TIS, 59(1), 1999, pp. 19-23
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
PLANT CELL TISSUE AND ORGAN CULTURE
ISSN journal
0167-6857 → ACNP
Volume
59
Issue
1
Year of publication
1999
Pages
19 - 23
Database
ISI
SICI code
0167-6857(1999)59:1<19:IPOPMU>2.0.ZU;2-Q
Abstract
A temporary immersion system for potato microtuber production was designed using 4-l vessels. This culture technique showed several advantages compare d to solid cultures: i.e., three fold increase in shoot length, more intern odes per plant and improved vigor. In the tuber induction stage, microtuber s can be induced at all plant nodes, indicating that the tuberization is no t restricted to specific regions. For both cultivars tested, Desiree and At lantic, an average of 3.1 and 2.8 tubers per single node cutting was achiev ed after 9 weeks in culture. The size and weight of the tubers were higher than on solid media. Scale up was performed with cv. Atlantic in 10-l polyc arbonate flasks and 12 units were mounted containing 150 single nodal cutti ngs each. An average of 2.6 tubers per inoculated cutting was obtained, wit h 1.3 g fresh weight per microtuber. Temporary immersion is a valuable opti on for potato microtuber production, as well as for shoot production during the planting season.