Structure and characterization of the murine p80 coilin gene, Coil

Citation
Ke. Tucker et al., Structure and characterization of the murine p80 coilin gene, Coil, J STRUCT B, 129(2-3), 2000, pp. 269-277
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF STRUCTURAL BIOLOGY
ISSN journal
1047-8477 → ACNP
Volume
129
Issue
2-3
Year of publication
2000
Pages
269 - 277
Database
ISI
SICI code
1047-8477(200004)129:2-3<269:SACOTM>2.0.ZU;2-#
Abstract
Cajal bodies (coiled bodies, CBs) are nuclear organelles of unknown functio n and are characterized by a wide variety of components including various b asal transcription and cell cycle proteins, the nucleolar proteins fibrilla rin and Nopp140, numerous small nuclear ribonucleoproteins, the survival mo tor neuron protein complex, and the marker protein, p80 coilin. To gain ins ight into the role of p80 coilin in CBs, we have cloned the murine gene Coi l and have mapped it to the distal portion of chromosome band 11D. The simi lar to 2.6-kb transcript is detectable in all tissues analyzed, with the hi ghest levels in brain and testis. Sequence analysis shows that, like its hu man counterpart, the mouse coilin gene is composed of seven exons and spans nearly 30 kb of genomic DNA. The predicted amino acid sequence reveals two conserved N- and C-terminal domains, and comparison with the Xenopus SPH-1 protein reveals that these three genes are indeed orthologous. These resul ts should facilitate gene disruption experiments aimed at creating a geneti c model system to study CBs. (C) 2000 Academic Press.