Normal blood pressure and plasma renin activity in mice lacking the renin-binding protein, a cellular renin inhibitor

Citation
C. Schmitz et al., Normal blood pressure and plasma renin activity in mice lacking the renin-binding protein, a cellular renin inhibitor, J BIOL CHEM, 275(20), 2000, pp. 15357-15362
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
0021-9258 → ACNP
Volume
275
Issue
20
Year of publication
2000
Pages
15357 - 15362
Database
ISI
SICI code
0021-9258(20000519)275:20<15357:NBPAPR>2.0.ZU;2-2
Abstract
In renal extracts, some renin is present as "high molecular weight renin," a heterodimeric complex of renin with the 46-kDa renin-binding protein (RnB P), also known as N-acyl-D-glucosamine a-epimerase. Because RnBP specifical ly inhibits renin activity, the protein was proposed to play an important r ole in the regulation of the renin-angiotensin system (RAS), Using gene tar geting, we have generated mice lacking RnBP and tested this hypothesis in v ivo. In particular, we analyzed biosynthesis, secretion, and activity of re nin and other components of the RAS in mice lacking RnBP, Despite extensive investigations, we were unable to detect any major effects of RnBP deficie ncy on the plasma and renal RAS or on blood pressure regulation. Contrary t o previous hypotheses, we conclude that RnBP does not play a significant ro le in the regulation of renin activity in plasma or kidney, However, RnBP k nockout mice excrete an abnormal pattern of carbohydrates in the urine, ind icating a role of the protein in renal carbohydrate metabolism.