Analysis of the actinobacterial insertion in domain III of the 23S rRNA gene of uncultured variants of the bacterium associated with Whipple's disease using broad-range and 'Tropheryma whippelii'-specific PCR

Citation
Hp. Hinrikson et al., Analysis of the actinobacterial insertion in domain III of the 23S rRNA gene of uncultured variants of the bacterium associated with Whipple's disease using broad-range and 'Tropheryma whippelii'-specific PCR, INT J SY EV, 50, 2000, pp. 1007-1011
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN journal
1466-5026 → ACNP
Volume
50
Year of publication
2000
Part
3
Pages
1007 - 1011
Database
ISI
SICI code
1466-5026(200005)50:<1007:AOTAII>2.0.ZU;2-3
Abstract
Heterogeneity in the 16S-23S rDNA spacer of uncultured 'Tropheryma whippeli i' has previously been reported. In this study, the hypervariable insertion in the 23S rDNA domain III characteristic for actinobacteria was analysed. The finding of a unique sequence virtually identical among the subtypes su pports the classification of 'T. whippelii' among the actinobacteria and th e concept of three subtypes rather than three subspecies, and provides an a lternative target for diagnostic assays.