ACTIVIN-A INHIBITS DIFFERENTIATION OF CHICK MYOGENIC CELLS IN-VITRO

Citation
M. Shiozuka et al., ACTIVIN-A INHIBITS DIFFERENTIATION OF CHICK MYOGENIC CELLS IN-VITRO, Zoological science, 14(2), 1997, pp. 327-330
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Zoology
Journal title
ISSN journal
0289-0003
Volume
14
Issue
2
Year of publication
1997
Pages
327 - 330
Database
ISI
SICI code
0289-0003(1997)14:2<327:AIDOCM>2.0.ZU;2-7
Abstract
We investigated the effect of activin A on the in vitro differentiatio n of primary myogenic cells isolated from chick embryonic breast muscl e. As judged by the morphology of the cells and accumulation of creati ne kinase, activin A inhibited myogenesis in a dose-dependent manner. The inhibitory activity was reversibly suppressed by folistatin. Activ in A also inhibited the expression of MyoD1. Our data also suggest tha t the inhibitory activity of activin A is exerted neither on prolifera tion of myoblasts or the growth of myotubes, but rather an early phase of differentiation following the withdrawal from cell cycle. A compar ison of actions of activin A and TGF-beta suggests that points of acti on is similar.