Expression, characterization, and localization of Rab26, a low molecular weight GTP-binding protein, in the rat parotid gland

Citation
S. Yoshie et al., Expression, characterization, and localization of Rab26, a low molecular weight GTP-binding protein, in the rat parotid gland, HISTOCHEM C, 113(4), 2000, pp. 259-263
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
HISTOCHEMISTRY AND CELL BIOLOGY
ISSN journal
0948-6143 → ACNP
Volume
113
Issue
4
Year of publication
2000
Pages
259 - 263
Database
ISI
SICI code
0948-6143(200004)113:4<259:ECALOR>2.0.ZU;2-9
Abstract
We investigated the expression of the genes encoding Rab proteins, low mole cular weight GTP-binding proteins, in the rat parotid gland by the use of r everse transcription-polymerase chain reaction, and detected cDNAs of Rab3D , Rab4, and Rab26. We further examined the characteristics and localization of Rab26 by western blotting and light and electron microscopic immunocyto chemistry. Western blotting using an antibody against the Rab26-specific, C -terminal peptide detected the His-tagged Rab26 protein as a single 27-kDa band. This band also displayed binding to [alpha-P-32]GTP. The fraction con taining secretory granule membranes in an acinar cell homogenate was immuno stained with the antibody. Supporting this, the immunocytochemical reaction for Rab26 was localized immediately around secretory granules in the acina r cells. The immunostaining disappeared from the acinar cells after treatme nt of rats with isoproterenol. These findings suggest that Rab26 participat es in the regulated secretion of granules and functionally belongs to the R ab3 group.