Determination of clozapine by capillary zone electrophoresis following end-column amperometric detection with simplified capillary/electrode alignment

Authors
Citation
Wr. Jin et al., Determination of clozapine by capillary zone electrophoresis following end-column amperometric detection with simplified capillary/electrode alignment, ELECTROPHOR, 21(7), 2000, pp. 1415-1420
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
21
Issue
7
Year of publication
2000
Pages
1415 - 1420
Database
ISI
SICI code
0173-0835(200004)21:7<1415:DOCBCZ>2.0.ZU;2-V
Abstract
Capillary zone electrophoresis was employed for the determination of clozap ine using an end-column amperometric detection at a carbon fiber array micr odisk electrode with simplified capillary/electrode alignment. The optimum conditions of separation and detection are: Britton-Robinson butter, pH 2.0 (1.3 x 10(-2) mol/L total concentration of acids, 3.2 x 10(-3) mol/L NaOH) , 15 kV for separation voltage, 5 kV and 10 s for injection voltage and inj ection time, respectively. The limit of detection is 4.2 x 10(-7) mol/L or 1.2 fmole (signal to noise, S/N = 2). The relative standard deviation is 1. 4% for the migration time and 2.5% for the electrophoretic peak current. Th e method was applied to the determination of clozapine in human blood. The recovery of the method is between 94-104%.