Wr. Jin et al., Determination of clozapine by capillary zone electrophoresis following end-column amperometric detection with simplified capillary/electrode alignment, ELECTROPHOR, 21(7), 2000, pp. 1415-1420
Capillary zone electrophoresis was employed for the determination of clozap
ine using an end-column amperometric detection at a carbon fiber array micr
odisk electrode with simplified capillary/electrode alignment. The optimum
conditions of separation and detection are: Britton-Robinson butter, pH 2.0
(1.3 x 10(-2) mol/L total concentration of acids, 3.2 x 10(-3) mol/L NaOH)
, 15 kV for separation voltage, 5 kV and 10 s for injection voltage and inj
ection time, respectively. The limit of detection is 4.2 x 10(-7) mol/L or
1.2 fmole (signal to noise, S/N = 2). The relative standard deviation is 1.
4% for the migration time and 2.5% for the electrophoretic peak current. Th
e method was applied to the determination of clozapine in human blood. The
recovery of the method is between 94-104%.