Quantitative assay of metronidazole by capillary zone electrophoresis withamperometric detection at a gold microelectrode

Citation
Wr. Jin et al., Quantitative assay of metronidazole by capillary zone electrophoresis withamperometric detection at a gold microelectrode, ELECTROPHOR, 21(7), 2000, pp. 1409-1414
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
21
Issue
7
Year of publication
2000
Pages
1409 - 1414
Database
ISI
SICI code
0173-0835(200004)21:7<1409:QAOMBC>2.0.ZU;2-A
Abstract
Capillary zone electrophoresis was employed for the determination of metron idazole using end-column amperometric detection with a gold microelectrode at a constant potential of -0.52V vs, saturated calomel electrode. To overc ome interference of oxygen in the solution, a deaeration injector and a dea eration protector at the detection cell were used. The optimum conditions o f separation and detection are 1.0 x 10(-3) mol/L potassium dihydrogen citr ate (KH2C6H5O7) for the buffer solution, 20 kV for the separation voltage, and 5 kV and 10 S for injection voltage and injection time, respectively. T he limit of detection is 6.0 x 10(7) mol/L or 0.78 fmole (S/N = 3). The rel ative standard deviation is 3.9% for the electrophoretic peak current. The method was applied to the determination of metronidazole in human urine.