Monitoring myoglobin by capillary zone electrophoresis with end-column amperometric detection

Citation
Wr. Jin et al., Monitoring myoglobin by capillary zone electrophoresis with end-column amperometric detection, ELECTROPHOR, 21(8), 2000, pp. 1535-1539
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
21
Issue
8
Year of publication
2000
Pages
1535 - 1539
Database
ISI
SICI code
0173-0835(200005)21:8<1535:MMBCZE>2.0.ZU;2-I
Abstract
Capillary zone electrophoresis was employed for the determination of myoglo bin in human urine using end-column amperometric detection with a carbon fi ber microelectrode at a constant potential of 1.80 V vs. saturated calomel electrode (SCF). The optimum conditions of separation and detection are: 3. 73 x 10(-4) mol/L sodium diethyl malonyl urea (barbitone sodium), 1.34 x 10 (-4) mol/L HCl for the buffer solution, 20 kV for separation voltage, 5 kV and 5 s for injection voltage and injection time, respectively. The limit o f detection is 4.4 x 10(-8) mol/L or 84 amole signal to noise (S/N = 2). Th e relative standard deviation is 2.9% for the migration time and 2.5% for t he electrophoretic peak current. The method can be used for the determinati on of myoglobin in human urine. The samples can be directly injected and ne ed no pretreatment. The method is also rapid, less than 2 min, and has a re covery rate of 94-106%.