Capillary zone electrophoresis was employed for the determination of myoglo
bin in human urine using end-column amperometric detection with a carbon fi
ber microelectrode at a constant potential of 1.80 V vs. saturated calomel
electrode (SCF). The optimum conditions of separation and detection are: 3.
73 x 10(-4) mol/L sodium diethyl malonyl urea (barbitone sodium), 1.34 x 10
(-4) mol/L HCl for the buffer solution, 20 kV for separation voltage, 5 kV
and 5 s for injection voltage and injection time, respectively. The limit o
f detection is 4.4 x 10(-8) mol/L or 84 amole signal to noise (S/N = 2). Th
e relative standard deviation is 2.9% for the migration time and 2.5% for t
he electrophoretic peak current. The method can be used for the determinati
on of myoglobin in human urine. The samples can be directly injected and ne
ed no pretreatment. The method is also rapid, less than 2 min, and has a re
covery rate of 94-106%.