A new capillary electrophoresis end-column amperometric detection system without the need for capillary/electrode alignment

Jin, WR Xu, Q Li, W

Wr. Jin et al., A new capillary electrophoresis end-column amperometric detection system without the need for capillary/electrode alignment, ELECTROPHOR, 21(8), 2000, pp. 1527-1534

36

INGLESE

Article

Chemistry & Analysis

ELECTROPHORESIS

0173-0835 → ACNP

21

8

2000

1527 - 1534

ISI

0173-0835(200005)21:8<1527:ANCEEA>2.0.ZU;2-H

A self-aligning end-column amperometric detection system for capillary elec trophoresis was constructed. In this system, the electrode and capillary we re exchanged easily and the capillary/electrode alignment procedure is not required. Gold, gold/mercury amalgam, copper and carbon fiber could be used as the working electrode. The principle is in the use of two disk holders with the capillary and the electrode in the center, so that by inserting th e disk holders into a groove in the working electrode port, the capillary a nd the electrode are automatically aligned and the distance between the cap illary and the electrode is assured at 0.24 mm. The relative standard devia tion obtained using five different gold/mercury amalgam microdisk electrode s for determination of cysteine was 1.5% for the migration time and 3.3% fo r the electrophoretic peak current. The simple and convenient system was at tractive for the routine analysis by capillary electrophoresis with electro chemical detection. The system was applied to the determination of prometha zine hydrochloride in human serum.