Cloning and expression of beta,beta-carotene 15,15 '-dioxygenase

Citation
A. Wyss et al., Cloning and expression of beta,beta-carotene 15,15 '-dioxygenase, BIOC BIOP R, 271(2), 2000, pp. 334-336
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
271
Issue
2
Year of publication
2000
Pages
334 - 336
Database
ISI
SICI code
0006-291X(20000510)271:2<334:CAEOB1>2.0.ZU;2-U
Abstract
beta,beta-Carotene 15,15'-dioxygenase cleaves beta-carotene into two molecu les of retinal and is therefore the key enzyme in beta-carotene metabolism to vitamin A. In the present study, it was possible to enrich the chicken b eta,beta-carotene 15,15'-dioxygenase to such an extent that partial amino a cid sequence information could be obtained to design degenerate oligonucleo tides. With RT-PCR a cDNA fragment could be obtained and used subsequently in a radioactive screening of a chicken duodenal expression library. We clo ned the first eukaryotic beta,beta-carotene 15,15'-dioxygenase which symmet rically cleaves beta-carotene at the 15,15'-double bond. (C) 2000 Academic Press.