In vitro analysis of platelet concentrates stored in the presence of modulators of 3 ',5 ' adenosine monophosphate, and organic anions

Citation
Ml. Lozano et al., In vitro analysis of platelet concentrates stored in the presence of modulators of 3 ',5 ' adenosine monophosphate, and organic anions, TRANSFUS SC, 22(1-2), 2000, pp. 3-11
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology
Journal title
TRANSFUSION SCIENCE
ISSN journal
0955-3886 → ACNP
Volume
22
Issue
1-2
Year of publication
2000
Pages
3 - 11
Database
ISI
SICI code
0955-3886(200002/04)22:1-2<3:IVAOPC>2.0.ZU;2-Y
Abstract
The storage of conventional platelet concentrates (PCs) under standard bloo d bank conditions is limited to five days, in part because longer storage p eriods lead to increasing damage in platelet integrity and functionality. T he growing demand of PCs for clinical use, raises the interest to develop a gents that would potentially permit a more extended period of storage. We h ave evaluated and compared the in vitro quality of PCs treated with: (1) Mo dulators of levels of cAMP (PGE(1), foskolin, theophylline and isobutyl-met hyl-xanthine [IBMX]); and (2) organic anions that function as alternative s ubstrates of platelets (pyruvate and acetate). Platelet rich plasma (PRP) f rom pools (n = 6) of PCs was distributed into storage bags, and the agents to be tested were added, using saline as a control substance. PCs were stor ed at 22 degrees C with continuous agitation for up to 10 days. At 0, 5 and 10 days of storage, samples were analyzed for platelet counts, mean platel et volume (MPV), metabolic markers, and expression of glycoproteins (GPs). The addition of modulators of levels of cAMP, at the concentration used in the study, did not lead to substantial improvement in the parameters being evaluated, with respect to those in control units. The supplementation with organic anions, while not affecting the surface levels of GPs, favored the maintenance of metabolic values, such as pH, PCO2, and bicarbonate concent rations, as well as the preservation of MPV (p values < 0.05 respect to con trol units both at 5 and 10 days of storage). Our results indicate that whi le the use of modulators of levels of cAMP do not provide substantial benef it in the prevention of platelet storage lesions, organic anions have some advantageous effect in the storage promoted metabolic changes of PCs. These data might be considered when designing strategies to improve PC storage. (C) 2000 Elsevier Science Ltd. All rights reserved.