Liquid chromatography was used to follow the degradation of hexopyranosylat
ed cytosine nucleosides in buffers of acid, neutral and alkaline pH and of
constant ionic strength. The compounds were found to degrade by hydrolysis
to cytosine and/or by deamination to the corresponding uracil nucleosides.
Degradation in acid is influenced by the number of sugar hydroxyl groups, p
resence of sugar double bonds and the type of anomer. Stability of some of
the compounds was compared with that of related thymine nucleosides. Temper
ature studies support a unimolecular mechanism of hydrolysis at pH 1.22.