Influence of ring opening-closure equilibrium of oxanine, a novel damaged nucleobase, on migration behavior in capillary electrophoresis

Citation
T. Suzuki et al., Influence of ring opening-closure equilibrium of oxanine, a novel damaged nucleobase, on migration behavior in capillary electrophoresis, J CHROMAT A, 877(1-2), 2000, pp. 225-232
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
877
Issue
1-2
Year of publication
2000
Pages
225 - 232
Database
ISI
SICI code
0021-9673(20000428)877:1-2<225:IOROEO>2.0.ZU;2-2
Abstract
Oxanine (Oxa) is a novel damaged nucleobase which is generated from guanine by HNO2 or NO. As a fundamental study for detection of Oxa formed in vivo, capillary zone electrophoresis (CZE) and micellar electrokinetic chromatog raphy (MEKC) have been tested by changing the pH of the running buffer. At pH 7, CZE did not separate Oxa from seven other nucleobases, and MEKC separ ated Oxa but their peaks migrated close together. In both the techniques, a n extreme peak broadening occurred for Oxa around pH 9 and a good peak sepa ration was achieved at pH 12. The behavior of the Oxa peak is discussed in relation to the unique multistep acid-base equilibria of Oxa. (C) 2000 Else vier Science B.V. All rights reserved.