Screening for microorganisms with specific characteristics by flow cytometry and single-cell sorting

Citation
T. Katsuragi et Y. Tani, Screening for microorganisms with specific characteristics by flow cytometry and single-cell sorting, J BIOSCI BI, 89(3), 2000, pp. 217-222
Citations number
29
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
89
Issue
3
Year of publication
2000
Pages
217 - 222
Database
ISI
SICI code
1389-1723(200003)89:3<217:SFMWSC>2.0.ZU;2-K
Abstract
Flow cytometry used in combination with single-cell sorting is a powerful t echnique for the identification and isolation of microbial cells with parti cular characteristics, especially when such cells grow more slowly than oth er cells in a large heterogeneous population. Many applications of flow cyt ometry with cell sorting, originally used by specialists studying mammalian cells, have been modified so that microorganisms also can be evaluated. Th e methods can now be used more widely because of the increasing availabilit y of the expensive equipment. There are means for the fluorescence detectio n of a wide variety of properties, such as amounts of various cell componen ts, specific sequences of peptides and nucleotides, cell functions, and enz yme activities. From the extensive literature, representative reports of an assortment of uses of flow cytometry with cell sorting are reviewed in thi s article, intended to introduce the technique and its many advantages to m icrobiologists.