A transmembrane guanylyl cyclase (DAF-11) and Hsp90 (DAF-21) regulate a common set of chemosensory behaviors in Caenorhabditis elegans

Citation
Da. Birnby et al., A transmembrane guanylyl cyclase (DAF-11) and Hsp90 (DAF-21) regulate a common set of chemosensory behaviors in Caenorhabditis elegans, GENETICS, 155(1), 2000, pp. 85-104
Citations number
100
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Molecular Biology & Genetics
Journal title
GENETICS
ISSN journal
0016-6731 → ACNP
Volume
155
Issue
1
Year of publication
2000
Pages
85 - 104
Database
ISI
SICI code
0016-6731(200005)155:1<85:ATGC(A>2.0.ZU;2-G
Abstract
Caenorhabditis elegans daf-11 and daf-21 mutants share defects in specific chemosensory responses mediated by several classes of sensory neurons, indi cating that these two genes have closely related functions in an assortment of chemosensory pathways. We report that daf-11 encodes one of a large fam ily of C. elegans transmembrane guanylyl cyclases (TM-GCs). The cyclic GMP analogue 8-bromo-cCMP rescues a sensory defect in both daf-11 and daf-21 mu tants, supporting a role for DAF-11 guanylyl cyclase activity in this proce ss and further suggesting that daf-21 acts at a similar step, daf-11:gfp fu sions are expressed in five identified pairs of chemosensory neurons in a p attern consistent with most daf-11 mutant phenotypes. We also show that daf -21 encodes the heat-shock protein 90 (Hsp90), a chaperone with numerous sp ecific protein targets. We show that the viable chemosensory-deficient daf- 21 mutation is an unusual allele resulting from a single amino acid substit ution and that the daf-21 null phenotype is early larval lethality. These r esults demonstrate that cGMP is a prominent second messenger in C. elegans chemosensory transduction and suggest a previously unknown role for Hsp90 i n regulating cGMP levels.