Monitoring pyridoxine by capillary zone electrophoresis with electrochemical detection

Authors
Jin, WR Zhang, J
Citation
Wr. Jin et J. Zhang, Monitoring pyridoxine by capillary zone electrophoresis with electrochemical detection, ELECTROANAL, 12(6), 2000, pp. 465-467
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ELECTROANALYSIS
ISSN journal
1040-0397 → ACNP
Volume
12
Issue
6
Year of publication
2000
Pages
465 - 467
Database
ISI
SICI code
1040-0397(200004)12:6<465:MPBCZE>2.0.ZU;2-9
Abstract
Capillary zone electrophoresis was employed for the determination of pyrido xine using end-column amperometric detection with a carbon fiber microelect rode at a constant potential of 0.72 V (vs. SCE). The optimum conditions of separation and detection are 1.0 x 10(-2) mol/L NH4Cl-1.0 x 10(-2) mol/L N H3 for the buffer solution, 10 kV for the separation voltage, 5 kV and 10 a for the injection voltage and the injection time, respectively. The limit of detection is 1.2 x 10(-6) mol/L or 3.5 fmol (S/N=2). The relative standa rd deviation is 1.3 % for the migration time and 3.4% for the electrophoret ic peak current. The method was applied to determining pyridoxine in human urine.