Expression and localization of basic fibroblast growth factor in diabetic rat prostate

Citation
Zj. Wang et al., Expression and localization of basic fibroblast growth factor in diabetic rat prostate, BJU INT, 85(7), 2000, pp. 945-952
Citations number
41
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Urology & Nephrology
Journal title
BJU INTERNATIONAL
ISSN journal
1464-4096 → ACNP
Volume
85
Issue
7
Year of publication
2000
Pages
945 - 952
Database
ISI
SICI code
1464-4096(200005)85:7<945:EALOBF>2.0.ZU;2-D
Abstract
Objective To assess fibroblast growth factor-2 (FGF2/bFGF), which is import ant in the development and maintenance of the normal prostate and in the de velopment of human benign prostatic hyperplasia (BPH) and prostatic carcino ma, in an animal model of experimentally induced diabetes. Materials and methods Using Western blotting and immunohistochemical analys es, the expression of FGF2 in prostates from several groups of rats was inv estigated. Rats had diabetes for 8 or 16 weeks (induced by intravenous inje ction with 65 mg/kg streptozotocin); rats were also treated with insulin (s tarting 8 weeks after the induction of diabetes, for 8 weeks), and two furt her groups acted as age-matched control rats. Immunohistochemical markers f or smooth muscle (alpha-actin) and epithelium (cytokeratin) were used to di stinguish different cell types in adjacent prostatic sections. Results Diabetic rats had smaller prostates and lower serum testosterone le vels than their controls; insulin treatment of diabetic rats increased pros tatic size and testosterone levels. As shown by Western blotting, diabetes caused greater FGF2 expression than in controls, whereas reverse-transcript ase polymerase chain reaction studies showed similar levels of prostatic FG F-2 mRNA in all groups. Immuno-histochemical studies showed that FGF-2 was expressed in both stromal and epithelial components of the rat prostate. Fu rthermore, although the expression of FGF2 was higher in epithelial than st romal cells in control prostates, it was distributed uniformly in the diabe tic prostate. Conclusion The differences in the level of expression and pattern of distri bution of FGF2 suggests a potential role for FGF2 in the changes observed i n prostatic growth in diabetic rats.