The changes in body weight and plasma metabolite levels during leptin injection are caused by the reduction of food intake in sheep

Citation
T. Tokuda et al., The changes in body weight and plasma metabolite levels during leptin injection are caused by the reduction of food intake in sheep, ANIM SCI, 70, 2000, pp. 343-348
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Animal Sciences
Journal title
ANIMAL SCIENCE
ISSN journal
1357-7298 → ACNP
Volume
70
Year of publication
2000
Part
2
Pages
343 - 348
Database
ISI
SICI code
1357-7298(200004)70:<343:TCIBWA>2.0.ZU;2-0
Abstract
Leptin is thought to act on the central nervous system as an important regu lator of food intake and body weight in mice. To examine the actions of lep tin in sheep, we injected recombinant mouse leptin into the lateral cerebra l ventricle of ewes. Six ewes were intracerebroventricularly cannulated and given free access to forage and wafer. The ewes were injected with phospha te-buffered saline (PBS) as a vehicle for 5 days through the cannula and th en with PBS + leptin for 7 days consecutively Although food intake and body weight did not change during PBS injection, the administration of leptin d ecreased food intake and body weight. Plasma glucose levels decreased and p lasma non-esterified fatty acid levels increased on the last few days of le ptin administration. Approximately 2 weeks after normal food intake was rec overed, the amount of diet was individually reduced in accordance with the recorded food intake during the leptin administration in each sheep. During food restriction, body weight and plasma metabolite concentrations were si milarly changed as during the leptin-administration period. These results i ndicate that leptin decreases food intake in sheep. Moreover, it is likely that the changes in body weight and plasma metabolites were mainly induced by the suppression of food intake during leptin injection.