Evidence of protein synthesis during resuscitation of ischemically damagedcanine pancreas by the two-layer method

Citation
S. Matsumoto et al., Evidence of protein synthesis during resuscitation of ischemically damagedcanine pancreas by the two-layer method, PANCREAS, 20(4), 2000, pp. 411-414
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
PANCREAS
ISSN journal
0885-3177 → ACNP
Volume
20
Issue
4
Year of publication
2000
Pages
411 - 414
Database
ISI
SICI code
0885-3177(200005)20:4<411:EOPSDR>2.0.ZU;2-Q
Abstract
The two-layer method, which supplies sufficient oxygen to the canine pancre as graft and allows adenotriphosphate synthesis within the graft, resuscita tes the ischemically damaged pancreas graft during mild hypothermic (20 deg rees C) preservation, but the mechanisms are unknown. The purpose of this s tudy was to determine whether protein synthesis was performed on ischemical ly damaged pancreas graft during preservation by the two-layer method in a canine autotransplantation model. The pancreas,grafts subjected to 90 minut es of warm ischemia were preserved by the two-layer method (perfluorochemic al/University of Wisconsin solution) at 20 degrees C for 5 hours. Graft via bility was judged from graft survival after autotransplantation. DNA, RNA, and protein synthesis was quantitated by determining incorporation of triti ated thymidine, tritiated uridine, and tritiated leucine, respectively, dur ing preservation. Significant increases in RNA and subsequent protein synth eses were observed during preservation by the two-layer method. In contrast , DNA synthesis did not follow RNA and protein synthesis. We conclude that protein is synthesized in the process of post ischemic cellular recovery of the pancreas graft during mild hypothermic preservation by the two-layer m ethod.