A putative enolpyruvyl transferase gene involved in nikkomycin biosynthesis

Citation
B. Lauer et al., A putative enolpyruvyl transferase gene involved in nikkomycin biosynthesis, J ANTIBIOT, 53(4), 2000, pp. 385-392
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF ANTIBIOTICS
ISSN journal
0021-8820 → ACNP
Volume
53
Issue
4
Year of publication
2000
Pages
385 - 392
Database
ISI
SICI code
0021-8820(200004)53:4<385:APETGI>2.0.ZU;2-V
Abstract
The nikO gene encoding a putative enolpyruvyl transferase has been identifi ed within the Streptomyces tendae Tu901/8c nikkomycin gene cluster. nikO en codes a deduced protein of 471 aminoacid residues which exhibits significan t sequence similarity to UDP-N-acetyl-glucosamine enolpyruvyl transferase a nd 5-enol-pyruvylshikimate 3-phosphate synthase from various origin. The ni kO gene was inactivated by inserting a kanamycin resistance cassette; the m utant did not produce biologically active nikkomycins I, J, X, and Z nor th e nucleoside moieties, nikkomycins C-x and C-z, but accumulated the novel c omponent RT 2.0. RT 2.0 has been isolated from culture filtrate and its str ucture was determined by using mass spectrometry and NMR analyses as ribofu ranosyl-4-formyl-4-imidazolone which represents a novel nucleoside. The put ative activity of the nikO gene product in nikkomycin biosynthesis will be discussed.