Isolation and characterization of alkane-utilizing Nocardioides sp strain CF8

Citation
N. Hamamura et Dj. Arp, Isolation and characterization of alkane-utilizing Nocardioides sp strain CF8, FEMS MICROB, 186(1), 2000, pp. 21-26
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
0378-1097 → ACNP
Volume
186
Issue
1
Year of publication
2000
Pages
21 - 26
Database
ISI
SICI code
0378-1097(20000501)186:1<21:IACOAN>2.0.ZU;2-A
Abstract
A butane-utilizing bacterial strain CF8 was isolated and identified as a me mber of the genus Nocardioides from chemotaxonomic and 16S rDNA sequence an alysis. Strain CF8 grew on alkanes ranging from C-2 to C-16 in addition to butane and various other substrates including primary alcohols, carboxylic acids, and phenol. Butane degradation by strain CF8 was inactivated by ligh t, a specific inactivator of copper-containing monooxygenases. The unique t hermal aggregation phenomenon of acetylene-binding polypeptides was also ob served for strain CF8. These results suggest that butane monooxygenase in s train CF8 is a third example of the copper-containing monooxygenases previo usly described in ammonia oxidizers and methanotrophs. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. A ll rights reserved.