Comparative organization of cattle chromosome 5 revealed by comparative mapping by annotation and sequence similarity and radiation hybrid mapping

Citation
A. Ozawa et al., Comparative organization of cattle chromosome 5 revealed by comparative mapping by annotation and sequence similarity and radiation hybrid mapping, P NAS US, 97(8), 2000, pp. 4150-4155
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
0027-8424 → ACNP
Volume
97
Issue
8
Year of publication
2000
Pages
4150 - 4155
Database
ISI
SICI code
0027-8424(20000411)97:8<4150:COOCC5>2.0.ZU;2-8
Abstract
A whole genome cattle-hamster radiation hybrid cell panel was used to const ruct a map of 54 markers located on bovine chromosome 5 (BTA5), Of the 54 m arkers, 34 are microsatellites selected from the cattle linkage map and 20 are genes. Among the 20 mapped genes, 10 are new assignments that were made by using the comparative mapping by annotation and sequence similarity str ategy. A LOD-3 radiation hybrid framework map consisting of 21 markers was constructed. The relatively low retention frequency of markers on this chro mosome (19%) prevented unambiguous ordering of the other 33 markers. The le ngth of the map is 398.7 cR, corresponding to a ratio of approximate to 2.8 cR(5,000)/cM. Type I genes were binned for comparison of gene order among cattle, humans, and mice. Multiple internal rearrangements within conserved syntenic groups were apparent upon comparison of gene order on BTA5 and HS A12 and HSA22, A similarly high number of rearrangements were observed betw een BTA5 and MMU6, MMU10, and MMU15. The detailed comparative map of BTA5 s hould facilitate identification of genes affecting economically important t raits that have been mapped to this chromosome and should contribute to our understanding of mammalian chromosome evolution.