Characterization of newly developed SSLP markers for the rat

Citation
Tk. Watanabe et al., Characterization of newly developed SSLP markers for the rat, MAMM GENOME, 11(4), 2000, pp. 300-305
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MAMMALIAN GENOME
ISSN journal
0938-8990 → ACNP
Volume
11
Issue
4
Year of publication
2000
Pages
300 - 305
Database
ISI
SICI code
0938-8990(200004)11:4<300:CONDSM>2.0.ZU;2-J
Abstract
We have isolated more than 12,000 clones containing microsatellite sequence s, mainly consisting of (CA)n dinucleotide repeats, using genomic DNA from the BN strain of laboratory rat. Data trimming yielded 9636 non-redundant m icrosatellite sequences, and we designed oligonucleotide primer pairs to am plify 8189 of these. PCR amplification of genomic DNA from five different r at strains yielded clean amplification products for 7040 of these simple-se quence-length-polymorphism (SSLP) markers; 3019 markers had been mapped pre viously by radiation hybrid (RH) mapping methods (Nat Genet 22, 27-36, 1998 ). Here we report the characterization of these newly developed microsatell ite markers as well as the release of previously unpublished microsatellite marker information. In addition, we have constructed a genome-wide linkage map of 515 markers, 204 of which are derived from our new collection, by g enotyping 48 F-2 progeny of (OLETFxBN)F-2 crosses. This map spans 1830.9 cM , with an average spacing of 3.56 cM. Together with our ongoing project of preparing a whole-genome radiation hybrid map for the rat, this dense linka ge map should provide a valuable resource for genetic studies in this model species.