Decreased mRNA expression of TNF-alpha and IL-10 in non-stimulated peripheral blood mononuclear cells in myasthenia gravis

Citation
Wx. Huang et al., Decreased mRNA expression of TNF-alpha and IL-10 in non-stimulated peripheral blood mononuclear cells in myasthenia gravis, EUR J NEUR, 7(2), 2000, pp. 195-202
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurology
Journal title
EUROPEAN JOURNAL OF NEUROLOGY
ISSN journal
1351-5101 → ACNP
Volume
7
Issue
2
Year of publication
2000
Pages
195 - 202
Database
ISI
SICI code
1351-5101(200003)7:2<195:DMEOTA>2.0.ZU;2-I
Abstract
Myasthenia gravis (MG) is characterized by T cell-dependent autoantibodies to the acetylcholine receptor on the post-synaptic membrane of the neuromus cular junction. The production of autoantibodies is regulated by T cells vi a cytokines. To investigate the involvement of interferon gamma (IFN-gamma) , tumour necrosis factor alpha (TNF-alpha), interleukin-4 (IL-4) and interl eukin-10 (IL-10) in MG, the mRNA levels of these cytokines in peripheral bl ood mononuclear cells without stimulation in vitro were quantified by compe titive reverse transcription polymerase chain reaction technique. The mRNA level of TNF-alpha was significantly lower (P = 0.0004) in the overall grou p of MC patients compared with controls. IL-10 was also lower in MG patient s (P = 0.008), most markedly in non-thymectomized patients (P = 0.016). The re were no significant differences in IFN-gamma and IL-4 bt tween patients and healthy controls, but the mRNA levels of IL-4 in non-thymectomized pati ents was significantly lower than in controls (P = 0.02) and in thymectomiz ed patients (P = 0.03). These results, reflecting the in vivo expression pa ttern of these cytokines in the periphery, suggest an altered cytokine expr ession at the systemic level in MG.