Combination of electrophoretic techniques for comprehensive analysis of complex protein systems

Authors
Citation
T. Manabe, Combination of electrophoretic techniques for comprehensive analysis of complex protein systems, ELECTROPHOR, 21(6), 2000, pp. 1116-1122
Citations number
27
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
21
Issue
6
Year of publication
2000
Pages
1116 - 1122
Database
ISI
SICI code
0173-0835(200004)21:6<1116:COETFC>2.0.ZU;2-A
Abstract
Analysis of proteins in complex mixtures such as cell lysates is presently performed by two-dimensional polyacrylamide gel electrophoresis under denat uring conditions (denaturing 2-D PAGE) followed by extraction of proteins f rom gel pieces and structural analysis of the proteins. This type of protei n analysis is contributing tb the correlation of information stored in DNA sequences with the structure of the product polypeptides. However, denaturi ng 2-D PAGE has its own limitations and it is necessary to develop various methods of protein analysis to reconstruct the total structure and function of proteins in complex systems. This review article summarizes the work in our laboratory to explore proteins in human plasma combining various elect rophoretic techniques: nondenaturing and denaturing 2-D PAGE, capillary ele ctrophoresis, and agarose gel isoelectric focusing.