Wood formation in rolC transgenic aspen trees

Citation
C. Grunwald et al., Wood formation in rolC transgenic aspen trees, TREES, 14(5), 2000, pp. 297-304
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
TREES-STRUCTURE AND FUNCTION
ISSN journal
0931-1890 → ACNP
Volume
14
Issue
5
Year of publication
2000
Pages
297 - 304
Database
ISI
SICI code
0931-1890(200003)14:5<297:WFIRTA>2.0.ZU;2-X
Abstract
Wood formation and structure of 3-year-old 35S-rolC transgenic aspen (Popul us tremula L. x tremuloides Michx.) were compared with the situation in non -transformed control aspen trees. The transgenics are characterized by redu ced shoot growth and an earlier bud break. Their wood formation did not imm ediately follow bud break and leaf development but occurred after some dela y, about the same time as wood formation was initiated in the control trees . Quantitatively, the wood structure of the transgenics and of the non-tran sformed controls was not significantly different; therefore the dwarfism of the transgenics is very likely due to a reduction in cell number. Atypical formation of latewood led to the assumption that the differentiation of ce lls is decelerated. Additionally, cells lacked both secondary walls and nor mal lignification, and discoloration of the wood and the formation of tylos es were conspicuous in all transgenics. In contrast, they did not occur in the non-transformed control aspen trees. The observations are discussed in relation to the widely accepted auxin hypothesis. It is suggested that 35S- rolC transgenic aspen trees may be a useful model to study the regulatory m echanisms of wood formation.