The diversity of periodontal spirochetes by 16S rRNA analysis

Citation
Fe. Dewhirst et al., The diversity of periodontal spirochetes by 16S rRNA analysis, ORAL MICROB, 15(3), 2000, pp. 196-202
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ORAL MICROBIOLOGY AND IMMUNOLOGY
ISSN journal
0902-0055 → ACNP
Volume
15
Issue
3
Year of publication
2000
Pages
196 - 202
Database
ISI
SICI code
0902-0055(200006)15:3<196:TDOPSB>2.0.ZU;2-F
Abstract
The purpose of this study was to examine the diversity of spirochetes in th e subgingival pocket of multiple subjects with a range of periodontal condi tions, including two healthy, one adult periodontitis, three acute necrotiz ing ulcerative gingivitis, eight refractory periodontitis, and one human im munodeficiency virus (HIV) periodontitis. The 16S rRNA genes of spirochetes in plaque were amplified by polymerase chain reaction using spirochete sel ective primers. Over 500 clones were sequenced and subjected to phylogeneti c analysis. The sequences clustered into the 10 known cultivated Treponema species and into 47 as-yet-uncultivated Treponema species. Most of these Tr eponema species were identified From multiple clones and subjects. The huma n periodontal pocket harbors a highly diverse treponeme population. Of the cultivated species, Treponema denticola, Treponema maltophilum and Treponcm a sp. Smibert-3 were most commonly encountered in diseased subjects but rar ely in healthy subjects. Molecular tools based on the sequence data from th is study will allow determination of the prevalence of cultivable and uncul tivable treponemes in oral diseases.