Chondroitinase ABC promotes axonal regeneration of Clarke's neurons after spinal cord injury

Citation
Lw. Yick et al., Chondroitinase ABC promotes axonal regeneration of Clarke's neurons after spinal cord injury, NEUROREPORT, 11(5), 2000, pp. 1063-1067
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROREPORT
ISSN journal
0959-4965 → ACNP
Volume
11
Issue
5
Year of publication
2000
Pages
1063 - 1067
Database
ISI
SICI code
0959-4965(20000407)11:5<1063:CAPARO>2.0.ZU;2-I
Abstract
We examined whether enzymatic digestion of chondroitin sulfate (CS) promote d the axonal regeneration of neurons in Clarke's nucleus (CN) into a periph eral nerve (PN) graft following injury of the spinal cord. After hemisectio n at T11, a segment of PN graft was implanted at the lesion site. Either ve hicle, brain-derived neurotrophic factor (BDNF) or chondroitinase ABC was a pplied at the implantation site. The postoperative survival period was 4 we eks. Treatment with vehicle or BDNF did not promote the axonal regeneration of CN neurons into the PN graft. Application of 2.5 unit/ml chondroitinase ABC resulted in a significant increase (12.8%) in the number of regenerate d CN neurons. Double labeling with Fluoro-Gold and NADPH-diaphorase histoch emistry showed that the regenerated CN neurons did not express nitric oxide synthase (NOS). Our results suggest that CS is inhibitory to the regenerat ion of CN neurons following injury of the spinal cord. NeuroReport 11:1063- 1067 (C) 2000 Lippincott Williams & Wilkins.