Atheroprotective effect of estriol and estrone sulfate on human vascular smooth muscle cells

Citation
N. Kikuchi et al., Atheroprotective effect of estriol and estrone sulfate on human vascular smooth muscle cells, J STEROID B, 72(1-2), 2000, pp. 71-78
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN journal
0960-0760 → ACNP
Volume
72
Issue
1-2
Year of publication
2000
Pages
71 - 78
Database
ISI
SICI code
0960-0760(200001/02)72:1-2<71:AEOEAE>2.0.ZU;2-8
Abstract
In patients with atherosclerosis, fibrosclerotic focuses are induced by mul tiplication of vascular smooth muscle cells (VSMC), and they are regulated by cytokines and regulators. There have been few reports about the atheropr otective effect of estriol (E-3) Estrone sulfate (E-1-S) is the predominant estrogen of conjugated equiline estrogens, which is commonly used in hormo ne replacement therapy, but it should be hydrolyzed by steroid sulfatase (S TS) to enter the cells of target tissues. The purpose of this study was to detect STS in VSMC and to investigate whether E-3 and E-1-S have atheroprot ective effects like E-2. First, we detected the presence of STS mRNA in VSM C by in situ hybridization. We then examined the changes in the expression of mRNAs of cytokines, namely, PDGF-A chain, IL-1, IL-6 and TCF-beta, in VS MC, in the presence and absence of E-3 and estrogens. As a result, the expr ession of PDGF-A chain, IL-1 and IL-6 mRNAs was suppressed by E-3 (P < 0.05 VS control) significantly like E-1-S and E-2, but that of TGF-beta mRNA wa s not significantly affected by any estrogen. These results indicate that E -1-S can be hydrolyzed by STS in VSMC, and that E-3 may regulate the cytoki nes by suppressing the production of mRNAs. It is suggested that there is a possibility of E-1-S and E-3 having a direct effect on vessels in atheroge nesis. (C) 2000 Elsevier Science Ltd. All rights reserved.