Two distinct lineages of macaque gamma herpesviruses related to the Kaposi's sarcoma associated herpesvirus

Citation
K. Strand et al., Two distinct lineages of macaque gamma herpesviruses related to the Kaposi's sarcoma associated herpesvirus, J CLIN VIRO, 16(3), 2000, pp. 253-269
Citations number
62
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease
Journal title
JOURNAL OF CLINICAL VIROLOGY
ISSN journal
1386-6532 → ACNP
Volume
16
Issue
3
Year of publication
2000
Pages
253 - 269
Database
ISI
SICI code
1386-6532(200005)16:3<253:TDLOMG>2.0.ZU;2-B
Abstract
Background: KSHV, Kaposi's sarcoma-associated herpesvirus, is a necessary c ofactor for the development of Kaposi's sarcoma (KS). We have previously re ported KSHV-related DNA sequences in retroperitoneal fibromatosis (RF) tiss ue from two species of macaque. The putative herpesvirus was called RFHV fo r RF-associated herpesvirus. These data suggested that KSHV is a human repr esentative of a larger family of primate herpesviruses. Objective: To ident ify and characterize other members of a putative family of KSHV-related her pesviruses in macaques in order to obtain information on the evolutionary h istory of KSHV infection in humans. Study design: Lymphoid tissue cells and blood leukocytes from rhesus-, cynomolgus- and pigtailed-macaques were tes ted for the presence of unknown herpesviruses using degenerate primer-drive n PCR amplification. The sequences obtained were compared against known her pesvirus sequences. Results: We have identified new herpesvirus DNA sequenc es in each of the three macaque species. Sequence comparisons indicate that these new viruses are most related to each other and form a separate phylo genetic lineage within the gamma herpesviruses. Screening of PBMC from Indo nesian-origin quarantine animals suggests that these viruses (MGV, macaque gamma virus) are species-specific, and highly prevalent in the wild. They a re readily cultured in vivo, and share a common tissue tropism with the pre viously identified RFHV. Conclusions: MGV and RFHV represent two independen t introductions of an ancestral gamma herpesvirus into macaque precursors. (C) 2000 Elsevier Science B.V. All rights reserved.