Anti-K-ras ribozyme induces growth inhibition and increased chemosensitivity in human colon cancer cells

Citation
T. Funato et al., Anti-K-ras ribozyme induces growth inhibition and increased chemosensitivity in human colon cancer cells, CANC GENE T, 7(3), 2000, pp. 495-500
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER GENE THERAPY
ISSN journal
0929-1903 → ACNP
Volume
7
Issue
3
Year of publication
2000
Pages
495 - 500
Database
ISI
SICI code
0929-1903(200003)7:3<495:ARIGIA>2.0.ZU;2-5
Abstract
Colon cancer is one of the carcinomas that is resistant to a variety of the rapies. To develop a new therapy by regulating the activated K-ras gene in colon cancers, we prepared synthetic DNA encoding the ribozyme (catalytic R NA), and inserted it into an expression vector (LNCX) originated from a ret rovirus. Transfection of the vector into SW620 human colon cancer cells bro ught about significant suppression of K-ras mRNA synthesis and inhibition o f the cell growth. Studies in athymic mice, in which K-ras ribozyme-introdu ced SW620 cells were transplanted, also revealed a marked reduction of tumo r growth in vivo. Furthermore, the ribozyme-introduced tumors became more s ensitive to treatment with anti-cancer drugs such as cisplatin, adriamycin, 5-fluorouracil, vincristine, and etoposide. These data suggest that the po ssible efficacy of anti-K-ras ribozyme increases the chemosensitivity of hu man colon cancers as well as the inhibitory effect on the growth of human c olon cancers.