Immunohistochemical localization of C-type natriuretic peptide in the rat submaxillary salivary gland

Citation
Es. Cho et al., Immunohistochemical localization of C-type natriuretic peptide in the rat submaxillary salivary gland, ARCH ORAL B, 45(5), 2000, pp. 425-430
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
ARCHIVES OF ORAL BIOLOGY
ISSN journal
0003-9969 → ACNP
Volume
45
Issue
5
Year of publication
2000
Pages
425 - 430
Database
ISI
SICI code
0003-9969(200005)45:5<425:ILOCNP>2.0.ZU;2-X
Abstract
To define the localization and characteristics of C-type natriuretic peptid e (CNP) in the rat submaxillary gland, immunohistochemistry and gel permeat ion-high-performance liquid chromatography were used. Immunoreactive (IR)-C NP was localized in cells of the granular convoluted tubule, striated duct and endothelial cells of the capillary, where atrial natriuretic peptide (A NP) was colocalized in consecutive sections, but not in acini. Gland extrac ts co-eluted with synthetic CNP and its content was 60.3 +/- 4.9 pg/mg prot ein (n = 4). Molecular profiles of immunoreactive material showed two peaks corresponding to synthetic CNP(1-53) and CNP(1-22). These results indicate that CNP is colocalized with ANP in the duct and endothelial cells of the rat submaxillary gland. Therefore, CNP may have a physiological role in the submaxillary gland by interacting with ANP and/or other biologically activ e substances in the ducts and granular convoluted tubule cells. (C) 2000 El sevier Science Ltd. All rights reserved.