Anti-tumor effect of N-beta-alanyl-5-S-glutathionyl-dihydroxyphenylalanine(5-S-GAD), a novel anti-bacterial substance from an insect

Citation
N. Akiyama et al., Anti-tumor effect of N-beta-alanyl-5-S-glutathionyl-dihydroxyphenylalanine(5-S-GAD), a novel anti-bacterial substance from an insect, ANTICANC R, 20(1A), 2000, pp. 357-362
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ANTICANCER RESEARCH
ISSN journal
0250-7005 → ACNP
Volume
20
Issue
1A
Year of publication
2000
Pages
357 - 362
Database
ISI
SICI code
0250-7005(200001/02)20:1A<357:AEON>2.0.ZU;2-M
Abstract
Purpose: We examined the anti-tumor effect of 5-S-GAD, a novel potent inhib itor of protein tyrosine kinases, isolated from the flesh fly in order to i nvestigate the potential use of this compound as an anti-tumor agent. Metho ds: In vitro growth inhibition was evaluated using the alamarBlue assay kit . In vivo anti-tumor activity was evaluated by i.p. treatment of 5-S-GAD ag ainst xenografted melanoma (LOX-IMV1) and breast carcinoma (MDA-MB-435S) in nude mice. Results: Of 38 human cancer cell lines examined, this compound showed significant cytotoxicity toward two estrogen-negative breast carcino mas (MDA-MB-231 and MDA-MB-435S) and one malignant melanoma (LOX- IMV1) in vitro, indicating that it exhibits selective cytotoxicity to certain tumor cell lines. In accordance with its in vitro anti-tumor effect, 5-S-GAD was shown to significantly repress the growth of sensitive tumor cells in nude mice. Conclusion: These results indicate that 5-S-GAD is potentially useful to treat certain human cancer.