Systemic upregulation of CD40 and CD40 ligand mRNA expression in multiple sclerosis

Citation
Wx. Huang et al., Systemic upregulation of CD40 and CD40 ligand mRNA expression in multiple sclerosis, MULT SCLER, 6(2), 2000, pp. 61-65
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurology,"Neurosciences & Behavoir
Journal title
MULTIPLE SCLEROSIS
ISSN journal
1352-4585 → ACNP
Volume
6
Issue
2
Year of publication
2000
Pages
61 - 65
Database
ISI
SICI code
1352-4585(200004)6:2<61:SUOCAC>2.0.ZU;2-9
Abstract
Is is increasingly clear that the CD40 and CD40 ligand (CD40L) receptor-lig and pair mediates a crucial activation signal in both cell-mediated and hum oral immune responses. Here, we detected mRNA levels of CD40 and CD40L in n on-stimulated peripheral blood mononuclear cells in 46 patients with multip le sclerosis (MS) and 46 healthy controls by a competitive RT-PCR procedure allowing quantification without previous culture or antigenic stimulation. The levels of CD40 and CD40L mRNA were markedly increased in MS patients ( P<0.0001) compared with healthy controls. There was no difference between c linical MS subgroups or stage of disease. Our findings indicate that althou gh MS is on organ specific disorder, an increased signaling via the CD40 an d CD40L pathway may be present or the systemic level. The nature of this up regulation, whether primary or secondary to the organ-specific autoimmune r esponse, is yet to be determined. Since interference with CD40/CD40L is on effective way to interfere with autoimmune model diseases such as experimen tal autoimmune encephalomyelitis, it may be relevant to investigate further the role of these molecules in the pathogenesis of MS.