Ultrastructural and molecular characterization of Pneumocystis carinii isolated from a rhesus monkey (Macaca mulatta)

Citation
I. Durand-joly et al., Ultrastructural and molecular characterization of Pneumocystis carinii isolated from a rhesus monkey (Macaca mulatta), MED MYCOL, 38(1), 2000, pp. 61-72
Citations number
59
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MEDICAL MYCOLOGY
ISSN journal
1369-3786 → ACNP
Volume
38
Issue
1
Year of publication
2000
Pages
61 - 72
Database
ISI
SICI code
1369-3786(200002)38:1<61:UAMCOP>2.0.ZU;2-2
Abstract
High levels of heterogeneity have been observed among isolates of Pneumocys tis carinii derived from different mammalian host species. We report the ch aracterization of P. carinii isolated from a rhesus monkey (Macaca mulatta) , which was immunosuppressed as a result of infection with a chimeric simia n-human immunodeficiency virus (SHIVsbg). Histopathological examination sho wed evidence of severe P. carinii pneumonia with a large predominance of tr ophozoite forms. Alveolitis consisted of typical foamy, honeycomb exudate, with only a few alveolar macrophages. The lung inflammatory response was ra ther moderate without type-2 pneumocyte hyperplasia or collagenosis. P, car inii organisms were sometimes observed in the bronchiolar lumen. Ultrastruc turally, macaque-derived P; carinii was more similar to human- or rabbit-de rived parasites than to mouse-derived P, carinii. Molecular studies were ca rried out on the macaque-derived P, carinii DNA at two genetic loci: the ge nes encoding the mitochondrial large subunit ribosomal RNA (mt LSU rRNA) an d the mitochondrial small subunit ribosomal RNA (mt SSU rRNA), Comparison o f the DNA sequences with those from P. carinii isolated from eight other ho st species demonstrated that the macaque-derived P, carinii was genetically distinct at both loci, and was more closely related to human-derived P, ca rinii than to P, carinii derived from non-primate sources. We propose that macaque-derived P, carinii be named Pneumocystis carinii f.sp. macacae.