Regulation of cyclic nucleotide phosphodiesterase (PDE) by zein peptides

Citation
Y. Kokean et al., Regulation of cyclic nucleotide phosphodiesterase (PDE) by zein peptides, J JPN SOC F, 47(3), 2000, pp. 220-226
Citations number
26
Language
GIAPPONESE
art.tipo
Article
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI
ISSN journal
1341-027X → ACNP
Volume
47
Issue
3
Year of publication
2000
Pages
220 - 226
Database
ISI
SICI code
1341-027X(2000)47:3<220:ROCNP(>2.0.ZU;2-H
Abstract
We have prepared zein peptides and characterized their ability to regulate the activity of cyclic nucleotide phosphodiesterase (PDE) isozymes obtained from canine heart ventricle. Native-zein inhibited 42%, 18%, and 25% of th e activities of PDE1, PDE2, and PDE4, respectively. AT1 was prepared by par tial hydrolysis of the native-zein with 0.3 N HCl at 50 degrees C for 24 hr . The AT1 increased the activities of 63% and 40% of PDE1 and PDE4. AT1 was hydrolyzed with pepsin, chymotrypsin, thermolysin, and subtilisin. Pepsin- and chymotrypsin-treated AT1 increased the activity of PDE1 by 75%. Deamin ated-zein were prepared by mild HCl hydrolysis(with 0.2 N HCl at 55 degrees C for 5 hr). Deamidation of zein had no effect on the activities of PDE1, PDE3, and PDE4. However, a deaminated-zein fraction (DA-3) increased PDE2 a ctivity by 16%. From these results, it was con Armed that zein peptides had regulatory effects on PDE isozymes.